Förlagets beskrivning
"In situ" hybridization is used to reveal the location of specific nucleic
acids sequences on chromosomes or in tissues. Visualization of the location of
genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for
understanding the organization, regulation, and function of genes. It is a
therefore a core technique in all areas of biomedical research. This edition
has been thoroughly updated to contain protocols detailing the major techniques
of "in situ" hybridization currently in use in the late 1990s: "in situ"
hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and
hapten labelled); analysis using light and electron microscopes; whole mount
"in situ" hybridization; double detection of RNAs, and RNA plus protein; and
fluorescent "in situ" hybridization to detect chromosomal sequences. This book is intended for all biomedical researchers at any level who use
"in situ" hybridization. This edition contains protocols detailing the major techniques of "in situ"
hybridization in the late 1990s. It includes topics such as: "in situ"
hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and
hapten labelled); and analysis using light and electron microscopes.
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In Situ Hybridization: A Practical Approach
Bokrecensioner » In Situ Hybridization: A Practical Approach (The Practical Approach Series)
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